An Evaluation of Analytical Methods for the Detection of Secondary Oxidation Products in Dedifferentiated Plant Cultures

BREMNER D.H., MAGILL WJ. DEIGHTON N.& BENSON E.E. 1997. An evaluation of analytical methods for the detection of secondary oxidation products in dedifferentiated plant cultures.-Phyton (Horn, Austria) 37 (3): (39) (44). A range of techniques have been employed to detect and examine aldehydic products of lipid peroxidation in D. carota cultures. Emphasis is placed on the use of HPLC and LC-MS techniques to aid the identification and profiling of aldehydic dinitrophenyl hydrazone (DNPH)derivatives in dedifferentiated callus tissues. Using these approaches, malondialdehyde (MDA) and 4-hydroxynonenal (HNE) have been identified in cultures of D. carota. Using LC-MS, it was found that embryogenic cultures have high levels of HNE and lower levels of MDA compared to nonembryogenic cultures. Future studies will evaluate the role of these lipid peroxidation products in in vitro morphogenesis.